FIGURE

Fig. 4

ID

ZDB-FIG-241203-35

Publication

Zhang et al., 2024 - Generation of Zebrafish Maternal Mutants via Oocyte-Specific Knockout System

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Fig. 4

Schematics illustrating the construction of U6 sgRNA expression vector. All U6 vectors have a U6 promoter and a sgRNA scaffold. Digestion of BsmBI makes U6 vectors expose the overhangs recognizing the annealed sgRNA primer. Digestion of PstI and SalI can disrupt the vector undigested by BsmBI to improve positive cloning.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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