FIGURE

Fig. 2

ID

ZDB-FIG-241014-2

Publication

Yang et al., 2024 - Compromised COPII vesicle trafficking leads to glycogenic hepatopathy in zebrafish

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Fig. 2

Epistasis analysis indicates that the ATF4 pathway serves as the main mediator for liver steatosis in the sec31anju221 background. (A) Oil Red O (ORO) staining of 7 dpf zebrafish larvae. Livers are outlined with dashed lines. Scale bar: 100 μm. (B) Quantification of ORO staining in livers as shown in A. Each data point represents an individual animal with bars representing mean±s.e.m. Comparison between groups was undertaken using one-way ANOVA with Tukey's multiple comparisons test. For A,B, n=6 for the sec31anju221; atf4a/b DKO group, and n=12 for all other groups. a.u., arbitrary units. ns, not significant; *P<0.05; ****P<0.0001. (C) Gene set enrichment analysis (GSEA) of RNA-seq expression values from larvae livers showing distinct separation of different genotypes. NES, normalized enrichment scores. Statistical analysis was performed using an empirical phenotype-based permutation test embedded in GSEA. (D) Heatmap showing the expression levels of genes involved in lipogenesis and lipid droplet formation in livers from sec31anju221 and sec31anju221; atf4a/b DKO larvae. FC, fold change.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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