FIGURE

Fig. 5

ID

ZDB-FIG-240712-18

Publication

Cui et al., 2024 - Lens autophagy protein ATG16L1: a potential target for cataract treatment

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Fig. 5

The interaction between ATG16L1 and E3 ubiquitin ligase gigaxonin is weakened by CX50 overexpression. (A) HEK293 cells were transfected with indicated plasmids, and IP was analyzed with HA affinity gels and Western blotting. (B) ATG16L1 deletion constructs. (C) Immunoblots of immunoprecipitates from CX50-Flag with Atg16L1-HA, ATG16L1-M, ATG16L1-C, ATG16L1-△N, and ATG16L1-△C in HEK293T cells. WCL: whole cell lysis. (D) HEK293 cells were transfected with CX50-GFP, ATG16L1-HA, or gigaxonin-Flag for 24 h. ATG16L1-HA was then immunoprecipitated by anti-HA, and the associated gigaxonin and ATG16L1 were analyzed by Western blotting.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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