Fig. 2

Clodrosome effectively reduces the number of macrophages. We administered either 2 nL of the vehicle control (encapsome [En], n = 15) or clodrosomes (Clo, 2.125 mg/mL, n = 18) to Tg(mpeg1:mCherry) larvae, as illustrated in Panel (a), or left them untreated (Unt, n = 15). Subsequently, as depicted in Panel (b) (with a fluorescence image superimposed on a bright field image), we quantified the number of macrophages (expressing mCherry in red) in the tail region, located 200 μm from the turning point of the tail blood vessel. At 1 and 24 h post-injection, we employed an epifluorescence microscope with a Rhodamine filter cube to assess the macrophages. (c) Representative images illustrating a focal plane for each treatment at specified time intervals. Due to the low resolution of the original images, we globally enhanced the images by increasing brightness and contrast using Photoshop. Utilizing the z-axis, we tallied the number of macrophages. The scatter plot with standard error bars is presented in (d). The unpaired t-test was used to analyze the differences between treatments. The p-values were calculated using the online tool GraphPad by Dotmatics (https://www.graphpad.com/quickcalcs/ttest1/?Format=SEM). ***p < .0001; the remaining p-values are shown in the figure.