FIGURE

Figure 2

ID

ZDB-FIG-240405-84

Publication

Diana et al., 2024 - Differentiation and functioning of the lateral line organ in zebrafish require Smpx activity

Other Figures

All Figure Page

Back to All Figure Page

Figure 2

Smpx protein localization in the neuromast mechanosensory hair cells. Confocal Z-stacks of immunofluorescence on whole mount 120 hpf larvae. (A–C) Smpx (green) localizes in the most internal cells of the neuromast, namely the mechanosensory hair cells, while it is excluded from the outermost cells (red) of the transgenic line tg (sod1:sod1; hsp70:DsRed) and the presumptive accessory cell layers (white bracket) encompassed in between the outmost DsRed-positive cells and the inmost hair cells. (D–F) Smpx (red) co-localizes with acetylated tubulin (green) in the kinocilium, with Smpx showing a ‘beads-on-a-string’ distribution (arrowheads in D). In some cases, the kinocilium is not labeled by the anti-Smpx antibody (arrow in F). (A–F) Larvae were counterstained with DAPI (blue) to visualize cell nuclei. Lateral views, anterior to the left. Scale bars are 15 µm in A–C and 10 µm in D–F.

Expression Data

Gene:	smpx
Antibody:	Ab1-smpx
Fish:	WT os4Tg
Anatomical Terms:	neuromast hair cell neuromast hair cell kinocilium
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.