FIGURE

FIGURE 3

ID

ZDB-FIG-240311-8

Publication

Van Schoor et al., 2024 - TUBA4A downregulation as observed in ALS post-mortem motor cortex causes ALS-related abnormalities in zebrafish

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FIGURE 3

Zebrafish motor behavior deficits are induced by tuba8l2 knockdown. Zebrafish were subjected to a touch-evoked escape response (TEER) assay at 48 hpf after injection of different doses of ATG morpholino against tuba8l2 (0.160 mM, 0.125 mM, 0.050 mM), or a control morpholino (0.160 mM). In addition, non-injected embryos were included in the analysis. (a) Total distance for 0.160 mM (p < 0.0001), 0.125 mM (p = 0.0001) and 0.050 mM (p = 0.1936) compared to AMO control condition. (b) Average velocity for 0.160 mM (p < 0.0001), 0.125 mM (p < 0.0001) and 0.050 mM (p = 0.9814) compared to AMO control condition. (c) Maximal instant velocity 0.160 mM (p < 0.0001), 0.125 mM (p < 0.0001) and 0.050 mM (p = 0.9983) compared to AMO control condition. Kruskal-Wallis test with Dunn’s multiple comparisons panel (a) or one-way ANOVA with Dunnett’s multiple comparisons panels (b,c); N = 3 experiments; n = 10–15 zebrafish embryos per group per experiment; with each data point representing an individual zebrafish embryo. (d) Visual example of the tracking of an escape response in the AMO control condition using the TEER assay in zebrafish embryos at 48 hpf. ***p < 0.001; ****p < 0.0001. AMO, morpholino; hpf, hours post fertilization.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-tuba8l2
Knockdown Reagent:	MO1-tuba8l2
Observed In:	whole organism thigmotaxis
Stage:	Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Cell. Neurosci.