|
Alignment of expression trajectories across zebrafish, mouse and human reveals concordant and divergent stages of maturation. a Heatmap of hierarchically clustered neutrophil maturation stages across studies11,13,48–51 based on zebrafish neutrophil maturation signatures (M1ortho, M2ortho, M3ortho). The four aggregated zebrafish phases are highlighted by a dotted box. Column annotations on the top show dataset annotation and row annotations on the left show module membership of each gene. Panels display zebrafish kidney marrow (b), mouse (c) and human (d) bone marrow (BM) scRNA-seq data ordered by their own maturation trajectory10,12,14,52–54. Ordered from top to bottom, column annotations display dataset annotations, inferred trajectories per dataset with pseudotime, cell density (histograms) and quality-flagged bins (<= 3 cells; potentially less reliable), heatmaps for modules M1ortho-M3ortho, and author-provided annotations. Left side annotation bars show module membership of each gene orthologue. e, f Average cross-correlation lag between mouse and human datasets (from panels c, d) and the corresponding zebrafish gene.
|
