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Peroxisome number is reduced in the pex13 mutant zebrafish liver and is partially rescued by chloroquine treatment. (A) Peroxisomes detected in wild type (WT) or pex13 mutant (pex13−/−) liver sections either stained with endogenous Abcd3 (upper row) or obtained from a transgenic zebrafish expressing RFP-SKL (lower row), at 5 dpf with and without CQ treatment for 24 h. (B) Quantification of images in (A). Relative abundance and colocalization of RFP-SKL-positive particles or Abcd3-positive particles in livers of WT and pex13−/− with and without CQ treatment were compared (n = 8; approximately 30–40 cells per liver section). Error bars represent standard deviation. (C) Representative images and their quantification from the liver sections of WT or pex13−/− with and without CQ treatment after staining with Oil red-O (ORO). Asterisks denote p-values; *p < 0.05. Scale bar: 10 μm (A), 40 μm (C).
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