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Fig. 1

ID

ZDB-FIG-230918-25

Publication

Gomes et al., 2023 - Protective effects of 24-epibrassinolide against the 6-OHDA zebrafish model of Parkinson's disease

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Fig. 1

Fig. 1. Exposure and analysis timeline. Fertilised eggs were collected at ∼2 h post-fertilization (hpf) and allowed to develop up to 24 hpf. At this point, the chorion was removed by pronase treatment and at 48 hpf animals were exposed for 72 h to 250 μM 6-OHDA, concomitantly to 24-EPI at different concentrations (0.01, 0.1 and 1 μM) and to 1 μM 24-EPI. Throughout the experimental period, developmental effects were assayed and, at the end of the exposure period, behavioural, oxidative-stress related parameters and TH-immunofluorescent neurons were evaluated.

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