CD271 expression increases the immune cell recruitment in vivo and in vitro a Representative images of mock and CD271-overexpressing SCC13 cells (green) injected into WT (indicated as WT/SCC13 in the figure) or Tg(lysC:DcRed)nz50 (indicated as LysC/SCC13) zebrafish with or without the simultaneous treatment with the L-LME. In the Tg(lysC:DcRed2)nz50 zebrafish, leucocytes possess a red fluorescence. Scale bar = 500 µm. b and c cSCC metastases quantified and classified as Full metastases, Initial metastases, or In place. d Quantification of LysC-positive cells in zebrafish injected with mock or CD271-overexpressing cells e SCC13 cells were stained with Vybrant DIO Cell-Labeling Solution (green) and seeded in a 96-well plate coated with 1,5% of agar. cSCC spheroids were transduced by infection with mock or CD271 viral vectors. After 72 h, THP-1 cells were stained with Vybrant DII Cell-Labeling Solution (red) and co-cultured with cSCC spheroids. Pictures were made after 24 and 72 h from the THP-1 seeding. Scale bar = 100µm. f The invasion area of THP-1 was measured by ImageJ software. Results are shown as the mean percentage of three independent experiments (minimum of 20 injected zebrafish/condition). Statistical analysis was performed using the two-way ANOVA and multiparametric T-test. **0.001 < p < 0.01, ****p < 0.0001
|
