FIGURE

Figure 5

ID

ZDB-FIG-230630-39

Publication

Kim et al., 2023 - Mechanism of Bisphenol F Affecting Motor System and Motor Activity in Zebrafish

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Figure 5

Toxic effects of BPF on motor axons and neurons of zebrafish larvae at 3 dpf. (A–C) Lateral views of the spinal cord of Tg(Olig2:dsRed) at 3 dpf. (D–F) Lateral views of the spinal cord of Tg(Hb9:mCherry) at 3 dpf. The motor neurons and interneurons expressed mCherry fluorescence. (G–I) Transverse section images of spinal cord of Tg(Hb9: GFP) zebrafish. The dead neurons (red spots) were confirmed through TUNEL assay. (J) The graph represents the length of olig2 positive motor axons. n = 12 for each concentration. (K) The graph represents the number of axonal branches per motor axon. n = 12 for each concentration. (L) The graph represents the number of Hb9 positive motor neurons. n = 15 for each concentration. Data are presented as means ± SD. n.s.—not significant, *** p < 0.001. (A–C) Scale bar, 100 µm. (D–F) Scale bar, 50 µm. ( G–I) Scale bar, 20 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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