Fig. 5

Dynamics of expression of proteins and mRNA involved in definition of the somite boundary during somitogenesis.

a–l series of fixed tg(her1:her1-venus) heterozygous embryos at the 7-somite stage stained Her1-venus protein (yellow) and dpERK (cyan) with ripply1 (magenta; a–c) and ripply2 (magenta; g–i) nascent mRNA. Magnified images of individual channels surrounded by white dotted squares are shown in the right panel. Phase was estimated by the relative position of Her1-venus stripes and anterior end of the dpERK signal gradient (a; n = 10, b; n = 8, c; n = 12, g; n = 9, h; n = 7, i; n = 9). Quantified signal intensity of (a–c, g–i) was plotted in (d–f, j–l) with anterior toward the left. m Schematic illustration of dynamics of Her1, Tbx6 proteins, and ripply1 and ripply2 transcription during zebrafish somitogenesis. n, o Double staining of Tbx6 protein (Blue) with Ripply1-V5 (n; green) or Ripply2-V5 (o; green). ripply1^v5/+ and ripply2^v5/v5 embryos were fixed at the 6–7 somite stage (n; n = 8, o; n = 9). Magnified images of individual channels surrounded by white dotted squares are shown right. Areas surrounded by a yellow dotted line indicate the most posterior domain of Ripply protein expression. Scale bar indicates 100 μm.