FIGURE

Fig. 5

ID

ZDB-FIG-230322-40

Publication

Tyrkalska et al., 2022 - Silica crystals activate toll-like receptors and inflammasomes to promote local and systemic immune responses in zebrafish

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Fig. 5

Fig. 5. Silica crystals signal through the canonical inflammasome in zebrafish. SiO₂ crystals or PBS were injected in the hindbrain ventricle (HBV) of 2 dpf Tg(lyz:DsRED2) (A) and wild type (B-M) larvae in the presence of either DMSO or the caspase-1 inhibitor VX-765 (VX). Neutrophil recruitment and number were analyzed at 6, 12 and 24 hpi by fluorescence microscopy (A), while the transcript levels of the indicated genes (B-L) were analyzed at 12 hpi by RT-qPCR in larval head and tail. Caspase-1 activity levels were analyzed at 24 hpi in larval head and tail (M). Each dot represents one individual and the mean ± S.E.M. for each group is also shown. One representative RT-qPCR and caspase-1 activity experiment from three biological replicates is shown. P values were calculated using one-way ANOVA and Tukey multiple range test. ns, not significant, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Comp. Immunol.