Fig. 3.

gba1 mutant macrophages are bactericidal. (A) Bacterial burden measured by fluorescence in 3 dpi gba1^sa1621/+ incross larvae treated with 100 units/mL velaglucerase alfa or vehicle control after HBV infection with 100 to 150 Mm. Horizontal bars, means; ns, not significant; *P < 0.05 (one-way ANOVA with Tukey’s posttest). Representative of two independent experiments. (B) Percentage of infected larvae at 5 dpi after infection of the HBV with a single Mm bacterium. *P < 0.05 (Fisher’s exact test). Representative of two independent experiments. (C) Maximum intensity projection of pseudocolored representative confocal image of YFP-expressing macrophages stained with LysoTracker Red in the brain of 2 dpf gba1 mutant larvae at 6 h after infection (hpi) in the HBV. Arrows mark vacuolated macrophages which have not phagocytosed Mm. White arrowheads mark nonvacuolated macrophages which have not phagocytosed Mm. Yellow arrowheads mark nonvacuolated macrophages which have phagocytosed Mm. (Scale bar, 20 μm.) (D) Quantification of nonvacuolated and vacuolated macrophages that are recruited to HBV in gba1 mutant larvae from (C). Each point represents the total number of recruited nonvacuolated or vacuolated macrophages in each fish. Horizontal red bars, means. ****P < 0.0001 (Student’s unpaired t test). (E) Quantification of nonvacuolated and vacuolated macrophages with phagocytosed Mm from (C). Each point represents the total number of infected nonvacuolated or vacuolated macrophages in each fish. Horizontal red bars, means. ****P < 0.0001 (Student’s unpaired t test).