Fig. 7

Heart position in MZcarmil3 mutant embryos compared to WT embryos at ~30 hpf, assessed by staining for transcripts of myl7, the gene encoding cardiac myosin light chain, in blue. (A) Representative images illustrate observed patterns. (B) Quantification of the sum of the two altered patterns, termed “heart jogging,” in two different MZcarmil3 mutant embryo lines compared with WT embryos. WT embryos displayed the abnormal (to the right or center) heart jogging phenotype 0.25% of the time (N=784). This value was significantly higher in the mutant embryos: 8.6% for MZcarmil3^stl413 (N=198) and 5.8% for MZcarmil3^sa19830 (N=415). Error bars indicates standard error of proportion. P values were calculated from Fisher’s exact test calculated with GraphPad Prism. Asterisks indicate that for both mutants, compared with WT, p values were <0.0001.