Figure 3

Masculinization and infertility induced by pld6 deficiency in zebrafish. A) Schematic of zebrafish pld6 genome locus and the gRNA target. Red font indicates gRNA target sequences. B) RT‐qPCR analysis of pld6 expression in 22 dpf gonads of pld6‐null mutant. C) Morphological and anatomical analyses of wildtype and pld6‐null mutant. D) Sex ratio of wildtype and pld6 ^−/− groups. N represents analyzed individual number. E,F) Comparison of body length and body weight between wildtype and mutant. G) Comparison of fertilization rates between wildtype and mutant. H) Comparison of the GSI between wildtype males and mutant males. I) Anti‐vasa immunostaining and in situ hybridization of wildtype and mutant testis with probes of gsdf and insl3. Scale bar: 50 µm for Vasa, 200 µm for gsdf and insl3. N represents analyzed individual number. The data were expressed as mean ± SD. The P values in this figure were calculated by two‐sided t‐test. *P < 0.05; ***P < 0.001; and ns, no significant difference; RT‐qPCR, reverse‐transcription quantitative PCR; GSI, gonadosomatic index.