FIGURE

FIGURE 6

ID

ZDB-FIG-221220-6

Publication

Barrett et al., 2022 - Dynamic functional and structural remodeling during retinal regeneration in zebrafish

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FIGURE 6

Time-course of restoration of PKCα + ON bipolar neurons and their synaptic terminals following extensive lesion. Tissues were processed for indirect immunofluorescence and imaging after ERG recordings. (A) Control retina displaying characteristic ON-bipolar synaptic terminals within the deep (s4-6) layers of the inner plexiform layer (IPL). (B) Retinal tissue at 4 days post-injury (DPI), demonstrating loss of retinal structure, and of cell types including sws2:mCherry + blue cones and PKCα + ON bipolar neurons. (C–H) Regenerating retinas at 13 (C), 21 (D), 30 (E), 45 (F), 60 (G), and 90 DPI (H). Some features of IPL emerge by 13 DPI, but laminar fusions (LF) remain, and PKCα + ON bipolar axon terminals are not entirely localized to IPL sublaminae s4-6. In addition, the occasional ectopic PKCα + nucleus [EBP in panel (G)] is found in the ganglion cell layer. ONL, outer nuclear layer; INL, inner nuclear layer; scale bar (in A, applies to all) = 50 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Mol. Neurosci.