FIGURE
Fig. 2
- ID
- ZDB-FIG-221122-11
- Publication
- Treffy et al., 2021 - Neuroblastoma differentiation in vivo excludes cranial tumors
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Fig. 2
(A and B) Lateral view schematics of zebrafish embryos injected with membrane-localized mCherry-expressing SK-N-AS NB cells (empty or scrambled vector; n = 30 embryos) at 2 hpi (A) and 14 hpi (B), for aROI and pROI assays only. Black letters/numbers indicate sites of injection; red and blue letters/numbers indicate final locations of neuronal and non-neuronal cells, respectively. (C–F) Still images from Video S7 of an injected SK-N-AS cell undergoing differentiation and apoptosis in the aROI. (G and H) Representative 3D projections of neuronal (G) or non-neuronal (H) SK-N-AS cells (purple) with length measurements. (I) Scatter plot of neuronal (red) and non-neuronal (blue) maximal projection lengths and their segregation into aROI, pROI, and trunk groups (gray). (J) The ratio of the number of SK-N-AS cells at 48 and 72 hpi in comparison with 15 hpi (n = 21 embryos for aROI and n = 9 embryos for pROI). (K–N′) 3D projections of injected NEUROD1:eGFP-expressing SK-N-AS cells present in the aROI or pROI at 3 hpi (K–L′) and 8 hpi (M–N′). (O) The percentage of SK-N-AS cells expressing NEUROD1:eGFP in vitro (cultured cells) or in vivo in the aROI or pROI. (P) Normalized eGFP intensity of single NEUROD1:eGFP-expressing cells in the aROI at 3–8 hpi (n = 10 cells, 1 representative cell/embryo). Horizontal bars (I, J, O, and P) denote mean values, with 95% confidence intervals indicated. n.s. p > 0.05; ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001. Scale bar: (A and B) 100 μm; (C–F) 40 μm; (G, H, and K–N′) 15 μm. |
Expression Data
Expression Detail
Antibody Labeling
Phenotype Data
Phenotype Detail
Acknowledgments
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Reprinted from Developmental Cell, 56(19), Treffy, R.W., Rajan, S.G., Jiang, X., Nacke, L.M., Malkana, U.A., Naiche, L.A., Bergey, D.E., Santana, D., Rajagopalan, V., Kitajewski, J.K., O'Bryan, J.P., Saxena, A., Neuroblastoma differentiation in vivo excludes cranial tumors, 2752-2764.e6, Copyright (2021) with permission from Elsevier. Full text @ Dev. Cell