FIGURE

Figure 3

ID

ZDB-FIG-221118-102

Publication

Seo et al., 2022 - Tamalin Function Is Required for the Survival of Neurons and Oligodendrocytes in the CNS

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Figure 3

The loss of tamalin function causes cell death of oligodendrocyte lineage cells in the postembryonic zebrafish spinal cord to adulthood. The representative images are transverse sections of the spinal cord of post-embryonic zebrafish. The dorsal side is displayed at the top. (a–f) Immunolabeling of wildtype (a–c) and tamalin KO (d–f) zebrafish with anti-Olig2 antibody (green) and TUNEL staining (red). (j–o) Immunolabeling of wildtype (j–l) and tamalin KO zebrafish (m–o) with anti-PCNA (green) and anti-Olig2 (red) antibodies. Quantification of the number of TUNEL⁺ Olig2⁺ cells (g–i) and PCNA⁺ Olig2⁺ cells (p–r) at 1 mpf (g,p), 2 mpf (h,q), and 4 mpf (i,r). (** p = 0.002 **** p < 0.0001, n = 10 sections from five zebrafish). Scale bars: (a–l), 20 μm. KO, knockout; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling; PCNA, proliferating cell nuclear antigen; ns; no significance and mpf; months post fertilization.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tamalin^km12/+ (AB)
Observed In:	oligodendrocyte apoptotic process oligodendrocyte cell population proliferation spinal cord apoptotic process spinal cord oligodendrocyte
Stage Range:	Days 30-44 to Adult

Phenotype Detail

Acknowledgments

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