Fig. 6

Effect of EPI-X4 derivatives and AMD3100 on CXCL12-evoked Ca²⁺ mobilization and ERK and AKT signaling. (A) Inhibition of CXCL12-induced calcium release. BCR-ABL expressing murine pro/pre B cells were loaded with Indo-1 AM and incubated with inhibitors for 10 min. Baseline fluorescence signal was recorded for 30 s and calcium flux induced by stimulation with 100 ng/mL mCXCL12 (black arrow). Signal was recorded for additional 260 s. (B) Areas under the curves (AUC) was calculated after baseline subtraction. Data were represented as mean ± SEM (n = 3). (C) and (D) Inhibition of CXCL12-induced ERK and AKT phosphorylation. SupT1 cells were preincubated with indicated concentrations of compounds for 15 min in starvation medium and afterwards stimulated with 100 ng/mL CXCL12 for 2 min. Cells were then permeabilized and stained for phosphorylated ERK (C) or phosphorylated AKT (D) and analyzed in flow cytometry. Data were represented as mean ± SEM (n = 3). IC₅₀ values were determined by non-linear regression. ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001; ∗∗∗∗P > 0.0001; ns = not significant (one-way ANOVA in comparison to PBS control).