FIGURE

Fig. 3

ID

ZDB-FIG-221111-3

Publication

Lam et al., 2021 - Identification of a wide spectrum of ciliary gene mutations in nonsyndromic biliary atresia patients implicates ciliary dysfunction as a novel disease mechanism

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

Fig. 3. Knockout of pcnt, kif3b and ttc17 resulted in defective bile flow in mutant zebrafish. (a) Representative epifluorescence images and (b) line plot of pcnt (n = 51), kif3b (n = 55) and ttc17 (n = 56) mutant embryo groups at 5 days post-fertilization showing significantly lower accumulated PED6 integrated density in gallbladder (arrowhead) after incubation for 30 min (left), 1 h (middle) and 2 h (right) compared to wild type (n = 57). Data expressed as mean ± standard error of the mean (SEM). Results of pairwise t-test: ***, P < 0.001; **, P < 0.01, *, P < 0.05.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + CRISPR1-kif3b WT + CRISPR1-ttc17 WT + CRISPR2-pcnt
Knockdown Reagents:	CRISPR1-kif3b CRISPR1-ttc17 CRISPR2-pcnt
Observed In:	hepaticobiliary system process intrahepatic bile duct
Stage:	Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ EBioMedicine