Fig. 2

Senolytics ameliorate the skin inflammation phenotype of Spint1a-deficient larvae. (A) General workflow for the treatment with senolytics of the chronic skin inflammation zebrafish model (spint1a^−/−). (B) Representative images of neutrophil phenotype and quantification of the number of neutrophils out of the CHT of 3-dpf larvae with neutrophils labeled in red (lyz:DsRED2). (C) Representative images of skin phenotype and quantification of the keratinocyte aggregate foci at the skin. The violin plots with the median shown as a horizontal line show the distribution of DsRED2⁺ cells and keratinocyte aggregate foci, and are overlaid with the raw data, where each dot represents an individual. (D) The mRNA level of il1b, tnfa, cxcl8b.1 were determined by RT-qPCR and normalized against rps11 in fin folds (indicated at the scheme). The bars show the mean ± SEM of 20 pooled larvae (n = 20) and triplicate samples from 2 independent experiments (n = 2). ns, non-significant; * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001 according to Kruskal-Wallis followed by Dunn’s multiple comparison test. CHT: caudal hematopoietic tissue. Scale bars, 400 μm.