FIGURE 6

The gosh mutant chronic injury model possesses a small population of M1-like pro-inflammatory microglia/macrophages. At 3 wpf, control gosh sibling retinas display few and ramified tdTomato-expressing microglia/macrophages (A–B”, arrows). In contrast, gosh mutant retinas exhibit many ameboid microglia/macrophages in the ONL and outer segments layers, with only a few of these microglia/macrophages co-expressing GFP (C–D”, arrowheads). White boxes in Panels A and C show the area magnified in Panels (B,D). White arrows: tdTomato-positive, GFP-negative microglia/macrophages; arrowheads: tdTomato and GFP double-positive microglia/macrophages. Histogram displays the number of microglia/macrophages in the entire section of the retinas (E). Bars and lines indicate mean ± SEM, n = 11 per group. Black bars: total number of tdTomato-positive cells; purple bars: tdTomato and GFP double-positive microglia/macrophages. One-way ANOVA with Tukey’s multiple comparisons test was used for statistical analysis (*p < 0.05; ***p < 0.001).