Fig. 4

Transcriptome analysis of VAT of scd5^−/− and scd5^−/− DE

(A) Histogram of number of DEGs obtained from the comparative analysis between the six samples. Pink square indicates upregulated, green square indicates downregulated (n = 2).

(B) Venn analysis of DEGs. Each elliptical box represents different gene groups. Four genes sets were obtained by intersection. VAT-up-scd5^−/− (2017 genes which were upregulated in VAT sample of scd5^−/− compared to scd5^+/+, restored in VAT sample of scd5^−/− DE, and not significantly different in SAT samples of all three strains) , VAT-down-scd5^−/− (7,776 genes which were downregulated in the VAT of scd5^−/− compared to scd5^+/+, restored in the VAT of scd5^−/− DE, and have no significantly difference in the SAT of scd5^−/− and scd5^−/− DE), SAT-up-scd5^−/− (862 genes which were upregulated in the SAT of scd5^−/− compared to scd5^+/+ and restored in the SAT of scd5^−/− DE, and have no significantly difference in the VAT of all three strains), and SAT-down-scd5^−/− (497 genes which were downregulated in the SAT of scd5^−/− compared to scd5^+/+, restored in the SAT of scd5^−/− DE, and have no significantly difference in the VAT of scd5^−/− and scd5^−/− DE).