Fig. 3

Fig. 3. Effects of elevated temperature on testis and ovary morphology in adult AB and NA zebrafish exposed to 28°C and 36°C during sex differentiation. A) Percent of different germ cell developmental stages in testes of AB males (n_28°C = 3, n_36°C = 5), NA_ZZ males (n_28°C = 7, n_36°C = 7) and NA_ZW neomales (n_28°C = 7, n_36°C = 8). AStudent's t-test was used to compare the number of each germ cell type between temperature treatments. Abbreviations: type A undifferentiated spermatogonia (A_und), type A differentiated spermatogonia (A_diff), type B spermatogonia (B), spermatocytes (Spc), spermatids (Spd), and spermatozoa (Spz). B) Spermatozoa number per field for AB (n_28°C = 3, n_36°C = 5), NA_ZZ males (n_28°C = 7, n_36°C = 7) and NA_ZW neomales (n_28°C = 7, n_36°C = 8). A Student's t-test was used to compare the number of spermatozoa between different groups. Boxes include values between the lower and upper quartiles, the upper whisker = min (max(x), Q3 + 1.5 *IQR), the lower whisker = max (min(x), Q1 – 1.5 * IQR), where IQR = third quartile (Q3) – first quartile (Q1). The line inside the boxplots indicates the median. C) Percent of different germ cell developmental stages in ovaries of AB (n_28°C = 7, n_36°C = 4), and NA_ZW females (n_28°C = 6). Note: NA_ZW females at 36 °C were not available due to 100% masculinization in all families. A Student's t-test was used to compare the number of each germ cell type between the two temperatures. Abbreviations: primary growth (PG) oocytes, cortical alveolar (CA) oocytes, vitellogenic (Vtg) oocytes, postovulatory follicles (POFs), and atretic (A) oocytes. Data shown as mean ± SEM. * = P < 0.05; ** = P < 0.01; *** = P < 0.001; N.S. = not significant. For sample size in each group see Table S4. For stage descriptions see Supplementary Information and Fig. 4. For detailed information of zebrafish germ cells (Leal et al., 2009).