Fig. 1

Fig. 1. SF-Whrna is present at the connecting cilium, in the inner segment, and at the synapse of the adult zebrafish photoreceptor. (A) Strep/Flag-tagged Whrna (green signal) is detected at the base of the connecting cilium, partially overlapping with centrin (red signal), and in the photoreceptor inner segments localized in the outer nuclear layer (ONL). In addition, signal is observed in the outer plexiform layer (OPL), where the photoreceptor synaptic regions are localized. (B) The retinas of wild-type zebrafish were used as negative control. No anti-FLAG signal could be detected. (C) Immunohistochemical analysis of wild-type zebrafish retinas using antibodies directed against Whrna. Whrna (green signal) is detected at the base of the connecting cilium, where it partially colocalizes with the connecting cilium marker centrin (red signal), and in the OPL, where the photoreceptor synaptic regions are localized. Co-localisation of Whrna and centrin is indicated by arrowheads. ROS = rod outer segment, RPE = retinal pigment epithelium, COS = cone outer segment, ONL = outer nuclear layer, OPL = outer plexiform layer, INL = inner nuclear layer. Scalebars represent 20 μm in the overview images and 10 μm in the higher magnification images. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)