FIGURE

Fig. 4

ID

ZDB-FIG-220727-52

Publication

Russo et al., 2022 - In vitro evolution of myc-tag antibodies: in-depth specificity and affinity analysis of Myc1-9E10 and Hyper-Myc

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Fig. 4

Flow cytometry analysis of myc-tagged antibodies bound to cells.

(A) Histograms of Daudi cells stained with either Myc1-9E10 (top panel) or Hyper-Myc (bottom panel) in the presence or absence of primary anti-CD19 scFv myc-tagged antibody fragment. Histograms are shown for concentrations of 2 μg/mL of Myc1-9E10 or Hyper-Myc, or 6.32 μg/mL for the negative controls. (B) Sensitivity of detection of myc-tagged anti-CD19 scFv on Daudi cells followed by incubation with either Hyper-Myc (red) or Myc1-9E10 (black) in concentrations ranging from 6.32 ng/mL to 6.32μg/mL. Alive single cells were gated; APC-conjugated goat anti-mouse IgG Fc was used for visualisation, and the median of the APC signal was measured using a MACSQuant flow cytometer. Error bars represent the standard deviation of duplicates.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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