FIGURE

Fig. 3

ID

ZDB-FIG-220701-16

Publication

Mesika et al., 2022 - NGLY1 Deficiency Zebrafish Model Manifests Abnormalities of the Nervous and Musculoskeletal Systems

Other Figures

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Fig. 3

Mutation characterization in zebrafish- (A) Genotype screening in DNA extracted from zebrafish, Representative electrophoregrams indicating the sequences of WT, heterozygote and homozygote. (B) qPCR analysis quantification of ngly1 mRNA levels. t-test (ngly1 ^(+/+) n = 3, ngly1 ^(−/−) n = 3, ***p < 0.001). (C) WB analysis quantification of ngly1 protein levels. t-test (ngly1 ^(+/+) n = 3, ngly1 ^(−/−) n = 3, **p < 0.01). (D) WB analysis with an antibody against ZF ngly1; β-actin was used as a loading control.

Expression Data

Gene:	ngly1
Antibody:	Ab1-ngly1
Fish:	AB ngly1^{zf3678/zf3678} (AB)
Anatomical Term:	caudal fin
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ngly1^{zf3678/zf3678} (AB)
Observed In:	caudal fin
Stage:	Adult

Phenotype Detail

Acknowledgments

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