Fig. 3
- ID
- ZDB-FIG-220622-134
- Publication
- Leseigneur et al., 2022 - NAD kinase promotes Staphylococcus aureus pathogenesis by supporting production of virulence factors and protective enzymes
- Other Figures
- All Figure Page
- Back to All Figure Page
Bacterial growth was monitored at OD600nm in BHI broth at 37°C. (A) Percentage of growth of the S. aureus strain containing the empty vector (pSD1) and the ppnK knockdown strain (NADK sgRNA) exposed for 6 hr to increasing concentrations of H2O2 relative to the untreated condition. (B) Percentage of growth of the S. aureus strain containing the empty vector (pSD1) and the ppnK knockdown strain (NADK sgRNA) exposed for 6 hr to H2O2 and catalase alone or in combination, relative to the untreated condition. Data shown are representative of three independent experiments. Bars indicate the standard error of the means of biological replicates. Comparison of data was performed using one-way analysis of variance (ns: nonsignificant, *p<0.05, ***p<0.001, ****p<0.0001). (C) Bacterial cell death of the S. aureus strain containing the empty vector (pSD1) and the ppnK knockdown strain (NADK sgRNA) untreated or exposed for 30 min to 0.05 mg/mL lysostaphin and 5 mg/mL lysozyme. Bacterial permeability was assessed using the CellTox Green cytotoxicity assay. Bars indicate standard errors of the means of biological replicates. Comparison of data was performed using two-ways analysis of variance (*p<0.05, **p<0.01, ***p<0.001, ****p<0.0001). Data are representative of at least three independent experiments.
|