FIGURE

Fig. 5

ID

ZDB-FIG-220613-6

Publication

Li et al., 2022 - Both prokaryotes and eukaryotes produce an immune response against plasmids with 5'-GTTTGTT-3'

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Fig. 5

Core sequences protect prokaryotic cells from plasmid infection and mutation of the core sequences increases plasmid transformation efficiency. Transformation efficiencies of different mutant forms of pEGFP-N1 in E. coli DH5α (A), E. coli BL21 (B), and E. coli FastT1 (C). Transformation efficiencies of different mutant forms of pXT7-myh9a in E. coli DH5α (D) and E. coli FastT1 (E). F Transformation efficiencies of different sized forms of pXT7-myh9a with mutant core sequence in ori. When the core sequence in ori is mutated, the number of clones is reduced for the small size pXT7-myh9a but increased for the large plasmid. Sanger sequencing results showing that the ori sequence of pEGFP-N1 favors survival compared with the 5ʹ-GTTTGTT-3ʹ sequence (G), but the ori sequence of pXT7 my17 confers no survival advantage (H)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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