Effect of EPN in zebrafish embryos at 96 h post-fertilization (hpf). (a), Phenotypes of EPN-treated zebrafish embryos at serial concentrations of 62.5, 125, 250, and 500 μg/L; (b), survival rates (%) of EPN-treated zebrafish embryos at serial concentrations of 62.5, 125, 250, and 500 μg/L; (c), hatching rates (%) of EPN-treated zebrafish embryos at serial concentrations of 62.5, 125, 250, and 500 μg/L; (d), deformity rates (%) in the EPN-treated zebrafish embryos at the different concentrations of 62.5, 125, 250, and 500 μg/L. PE means pericardial edema, and YSE indicates yolk sac edema. BP means blood pooling. The scale bar represents 200 µm. A lowercase letter indicates a significant difference (p < 0.05).
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