FIGURE

Figure 7

ID

ZDB-FIG-220211-14

Publication

Carril Pardo et al., 2022 - A δ-cell subpopulation with pro-β cell identity contributes to efficient age-independent recovery in a zebrafish diabetes model

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Figure 7

Bihormonal cells can also arise in the pancreatic ducts.

(A–B) Whole mount immunodetection of GFP that highlights the ducts (green), mCherry (red) for β-cells and Sst (gray) on the entire pancreas of Tg(nkx6.1:eGFP); Tg(ins:NTR-P2A-mCherry) larvae at 17 dpf. (A) CTL larvae showing the main islet in the head and a few monohormonal endocrine cells (mCherry+ or Sst+) in the ductal GFP+ domain in the tail. The pancreatic tail is delineated by white dashed lines. (B) After treatment with NFP from 3 to 4 dpf, regenerating larvae display scattered bihormonal cells (red and gray) in the tail along the ducts. Stacks represent 3D projections of confocal images of the whole pancreas. (B’) Close-ups of two individual bihormonal cells in the tail (z-planes showing one unique optical section). (C) Quantification of Sst + mCherry + bihormonal cells based on confocal images. Mann-Whitney test, ****p < 0.0001. (See also Figure 7—source data 1).

Expression Data

Genes:	EGFP mCherry sst1.1
Fish:	ulg004Tg/ulg004Tg; ulg034Tg/ulg034Tg
Condition:	chemical treatment by environment: nifurpirinol
Anatomical Terms:	pancreas primordium pancreatic B cell insulin secreting cell
Stage:	14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ulg004Tg/ulg004Tg; ulg034Tg/ulg034Tg
Condition:	chemical treatment by environment: nifurpirinol
Observed In:	pancreatic B cell insulin secreting cell
Stage:	14-19 somites

Phenotype Detail

Acknowledgments

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