Fig. 1

A) Mating strategy and experimental design. B) Schematic of the human PSEN2 protein (adapted from https://www.alzforum.org/mutations/psen-2 with permission from FBRI LLC (Copyright © 1996–2020 FBRI LLC. All Rights Reserved. Version 2.7 –2020)). Amino acid residues are color-coded to indicate whether substitutions have been observed to be pathogenic (orange), non-pathogenic (green) or of uncertain pathogenicity (blue). The site of the human EOfAD mutation N141I, in the second transmembrane domain (TMD) is indicated by the red single-headed arrow. The aspartate residues critical for γ-secretase catalysis are indicated by a red double-headed arrow. C) Upset plot indicating the number of differentially expressed (DE) genes in each comparison of the psen2 mutant genotypes to wild type noting that only 2 genes appear to be DE in common between both comparisons. D) Mean-difference (MD) plot and E) volcano plot of DE genes in each comparison. Note that the logFC axis limits in D) and E) are constrained to -5 and 5 for visualization purposes.