Figure 2

Increased pressure causes chamber remodeling by 56 hpf. (A) Cardiac output (CO) at 40 hpf at increasing doses of vasopressin (control, 2.5, 5, 7.5, and 10 µm). (B) CO at 56 hpf at increasing doses of vasopressin (vaso). n = 5 fish per group. (C) Cardiac output at 40 and 56 hpf in control and 10 µm vaso-treated fish. Each colored dot represents an individual fish; orange-colored dots represent control fish, light blue represents mildly-affected fish, and purple represents severely-affected fish. n= 4 control, 8 vaso. (D) Rate of change for cardiac output with respect to time. (E) Stroke volume and (F) heart rate of control and 10 µm vaso-treated fish at 40 and 56 hpf. n = 11 control and 12 vaso-treated fish at 40 hpf, and 8 and 13 vasopressin-treated fish at 56 hpf. * indicates p < 0.05