Fig. 7
- ID
- ZDB-FIG-211118-89
- Publication
- Liu et al., 2021 - NudCL2 is an autophagy receptor that mediates selective autophagic degradation of CP110 at mother centrioles to promote ciliogenesis
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Knockdown of CP110 reverses the ciliary defects in NudCL2 morphants.
Embryos were injected with the indicated MOs and harvested at different times. a Immunoblot analysis of NudCL2 and CP110 expression. α-tubulin, a loading control. b–d Immunofluorescence of cilia with anti-acetylated-α-tubulin antibody in KVs at 6–8 somite stages (b). Scale bar, 10 µm. The cilia number per KV was calculated (c). Cilia length in KV was measured using ImageJ software (d). e–h Whole-mount in situ hybridization with cmlc2 probe (48 hpf) and foxa3 probe (72 hpf). Liver and pancreas are indicated by arrows and arrowheads, respectively. The percentage of the expression patterns of cmlc2 and foxa3 was calculated (g, h). The data are presented as means ± SD derived from at least three independent experiments. n, sample size. **P < 0.01 and ***P < 0.001 Student’s t-test. i Working model of NudCL2-mediated autophagic degradation of CP110 at mother centrioles to initiate ciliogenesis. See text for details. |
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| Stage: | Long-pec |
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| Stage Range: | 5-9 somites to Protruding-mouth |