FIGURE

Fig. 2.

ID

ZDB-FIG-210913-3

Publication

Lubin et al., 2021 - A versatile, automated and high-throughput drug screening platform for zebrafish embryos

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Fig. 2.

Validation of HSPC counts using the Athena Zebrafish Application to detect differences in stem cell populations due to age or genetics of zebrafish embryos. (A) Brightfield and fluorescent images of Tg(itga2b:GFP) embryos at 2-4 dpf and analysed for HSPC count (green) in the tail region (red). (B) HSPC counts at 2 dpf (n=48), 3 dpf (n=54) and 4 dpf (n=47), showing the increase in HSPC number over time. (C) Fluorescent images of the tail of rps14^+/+ and rps14^+/− Tg(itga2b:GFP) at 3 dpf following phz haemolytic stress for 24 h at 24 hpf alongside unstressed controls analysed for HSPC count (green) in the tail region (red). (D) HSPC count of the different conditions, showing no difference in HSPC count between unstressed rps14^+/− (n=27) and unstressed rps14^+/+ (n=22) embryos, but an increase in HSPC count in the phz-stressed rps14^+/+ embryos (n=29) compared with wild type, which does not occur in the phz-stressed rps14^+/− embryos (n=29), from triplicate experiments (Peña et al., 2020 preprint). Statistical analysis using unpaired t-tests. Error bars show mean±s.d. ns, P>0.05; **P<0.01. Scale bars: 500 µm. n refers to the number of embryos analysed for each condition. Cell counts give the Athena cell count for the example image shown.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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