FIGURE

Fig. 3

ID

ZDB-FIG-210913-14

Publication

Zhang et al., 2021 - Large-scale phenotypic drug screen identifies neuroprotectants in zebrafish and mouse models of retinitis pigmentosa

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Fig. 3

Confocal imaging of neuroprotective effects of confirmed lead drug compounds.

Representative 7 dpf in vivo confocal images of YFP-expressing rod cells in an ablated control (+Mtz), non-ablated control (-Mtz), and in Mtz-exposed retinas treated with lead compounds from 5 to 7 dpf. Whole retina 3D image stacks (~150 micron depth) show loss or preservation of YFP-expressing rod cells throughout the retina, zoomed images of a more limited stack in the dorsal-nasal quadrant (~12 micron depth) provide rod cell morphological detail. Lead compound abbreviations: WAR, Warfarin; CLO, Cloxyquin; CPO, Ciclopirox olamine; MIC, Miconazole; ZPT, Zinc pyrithione; DHA, Dihydroartemisinin; CHL, Chloroxine; CAL, Calcimycin; SUL, Sulindac; ART, Artemisinin; COR, Cortexolone; POS, positive control. Other abbreviations: CI, confidence interval; Mtz, Metronidazole; PTU, N-phenylthiourea.

Expression Data

Gene:	YFP
Fish:	mpv17^a9; gmc500Tg
Conditions:	chemical treatment by environment: (+)-artemisinin chemical treatment by environment: Calcimycin chemical treatment by environment: chloroxine chemical treatment by environment: ciclopirox olamine chemical treatment by environment: cloxiquine chemical treatment by environment: metronidazole chemical treatment by environment: metronidazole chemical treatment by environment: metronidazole chemical treatment by environment: miconazole chemical treatment by environment: sulindac chemical treatment by environment: warfarin chemical treatment by environment: zinc pyrithione
Anatomical Term:	retinal rod cell
Stage:	Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mpv17^a9; gmc500Tg
Conditions:	chemical treatment by environment: (+)-artemisinin chemical treatment by environment: Calcimycin chemical treatment by environment: chloroxine chemical treatment by environment: ciclopirox olamine chemical treatment by environment: cloxiquine chemical treatment by environment: metronidazole chemical treatment by environment: metronidazole chemical treatment by environment: metronidazole chemical treatment by environment: miconazole chemical treatment by environment: sulindac chemical treatment by environment: warfarin chemical treatment by environment: zinc pyrithione
Observed In:	retinal rod cell
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

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