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Fig. 7

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ZDB-FIG-210804-28

Publication

Fraher et al., 2021 - The endocannabinoid system and retinoic acid signaling combine to influence bone growth

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Fig. 7

Fig. 7. Combined sup-optimal doses of RA and ECS modulating chemicals is sufficient to influence bone development. Zebrafish were treated from 50 to 72 hpf with 60% combined doses of RA + RIMO, DEAB + OLE, or DEAB + WIN and mineralization of the opercle (black arrowhead) and opercle stem (white arrowhead) was assessed at 144 hpf with von Kossa stain (A–H). RA + RIMO showed no effect on mineralization (C,D). Both DEAB + OLE or DEAB + WIN treatments were sufficient to reduce mineralization (E–H). Measurements of the area of staining of the opercle stem support these observations (M, n = 9). The effects of combined doses on col10a1 expression was assessed at 72 hpf following treatment from 36 to 72 hpf (I–L). RA + RIMO showed no effect on col10a1 expression (J). Both DEAB + OLE or DEAB + WIN treatments were sufficient to reduce expression (K,L). Quantification of staining of the opercle supports the observed expression (N, n = 10).

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Reprinted from Molecular and Cellular Endocrinology, 529, Fraher, D., Mann, R.J., Dubuisson, M.J., Ellis, M.K., Yu, T., Walder, K., Ward, A.C., Winkler, C., Gibert, Y., The endocannabinoid system and retinoic acid signaling combine to influence bone growth, 111267, Copyright (2021) with permission from Elsevier. Full text @ Mol. Cell. Endocrinol.