FIGURE 6

Tg(dmrt3a:GAL4;UAS:tdTomato) injected with Kaloop plasmid reveal multiple inhibitory efferent cells innervating a single neuromast. (A) One-cell stage embryos of Tg(dmrt3a:GAL4;UAS:tdTomato) were injected with Kaloop plasmid to generate larvae with sparsely labeled tdTomato and eGFP inhibitory efferents. (B) Schematic overview of the area shown in (E). (C–D) Confocal images showing the view of sparsely labeled inhibitory efferent cells in their nuclei. A ROLE (tdTomato-positive; magenta) and a RELL (eGFP-positive; white) cell were identified (C) and their projections traced across the lateral line (D) dorsal view and (E) lateral view of the inhibitory efferent projections at the level of the ear. In this case, both efferent cells were connected to the same neuromast located on the ear (MI1) [(E’) depicts the MI1 neuromast at higher magnification]. Age of larvae: 5 dpf. ROLE, the rhombencephalic octavolateral efferent neuron; RELL, the rhombencephalic efferent neuron to the lateral line. (A) was partially created with BioRender.com