Seventy-two hours postfertilization behavioral results. (a) After 1 week of recovery, larvae exposed to the high concentration of BPA at 72 hpf displayed statistically increased startle responses relative to all other treatment groups (p < 0.001). (b) There was also a significant increase in positive OMRs (p < 0.001) in the high BPA group. (c) At the 2 week recovery time, larvae exposed to high BPA displayed significantly increased startle responses compared to low BPA exposed larvae (p = 0.003), though neither response was different from the vehicle control. (d) The percentage of larvae displaying a positive OMR was significantly increased in both the low BPA (p < 0.001) and high BPA (p = 0.001) treatment groups, with the greatest response observed in larvae exposed to low BPA. At each recovery point n = 30 for all treatment groups. Startle responses were analyzed using a one-way ANOVA performed separately for each age and treatment group; if significance was noted, a Tukey post hoc test was performed to identify differences. OMR was analyzed using a one-way ANOVA, followed by a post hoc multiple comparison (with a Bonferroni correction). All values are reported as mean ± SE. Different letters denote significance.
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