FIGURE

FIGURE 2

ID

ZDB-FIG-210520-10

Publication

Liu et al., 2021 - Mutation of Gemin5 Causes Defective Hematopoietic Stem/Progenitor Cells Proliferation in Zebrafish Embryonic Hematopoiesis

Other Figures

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FIGURE 2

The mutation was mapped to a point mutation in gene gemin5. (A) The alignment of WT (underlined) and NC_007132.7: g.36404684 C > T (gemin5⁰⁰⁸) sequences are listed. The mutation by ENU is indicated in red (a C to T nonsense mutation causes an immediate stop codon in gemin5 in mutant^cas008). (B) According to the stop codon in the genome, SMART software was used to predict the structure of Gemin5^WT and XP_001339880.4:p.Gln303_His1440del (Gemin5⁰⁰⁸) presumed protein. The molecular sizes of the presumed protein are indicated on the right. (C) WISH analysis with myb probe in the CHT region (at 6 dpf) of sibling and gemin5⁰⁰⁸ with transient transgenesis of vector (only plasmid backbone) or ubi: gemin5 (#1, The construction of the plasmid is shown below).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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