FIGURE

Fig. 5

ID
ZDB-FIG-210508-15
Publication
Laquatra et al., 2021 - HIF1α-dependent induction of the mitochondrial chaperone TRAP1 regulates bioenergetic adaptations to hypoxia
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Fig. 5

TRAP1 is overexpressed in pancreatic tumors and downregulates SDH activity.

a Western blot analysis and protein quantification of TRAP1 in wild-type pancreas and in pancreatic tumors driven by KRASG12D expression (1 year post-fertilization, ypf). Values were normalized for citrate synthase. b Analysis of TRAP1 mRNA expression in wild-type pancreas and in pancreatic tumors at 1 ypf. Values were normalized for expression of rpl13 and actin, used as housekeeping genes. The number of animals used in each condition is reported inside column bars. c Colorimetric assay (pink staining) of SDH activity in tissue slides of wild-type pancreas and pancreatic tumors with/without the TRAP1 specific inhibitor compound 5 (100 μM, 30 min pre-incubation). In (a, b), data are reported as average ±SEM of at least four different animals per condition with an unpaired two-tailed Student’s t test; asterisks indicate significant differences (*p < 0.05).
Expression Data
Gene:
Fish:
Anatomical Term:
Stage: Adult

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage: Adult

Phenotype Detail
Acknowledgments
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