FIGURE

Figure 1

ID

ZDB-FIG-210414-1

Publication

Sipka et al., 2021 - Damage-Induced Calcium Signaling and Reactive Oxygen Species Mediate Macrophage Activation in Zebrafish

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Figure 1

Tg(mfap4:mCherry-F/tnfa:GFP-F) reporter line allows imaging of macrophage M1-like activation at the wound in response to fin fold injury. (A) The caudal fin fold of Tg(mfap4:mCherry-F/tnfa:GFP-F) larvae were amputated at 3 dpf. Schedule of the recruitment and M1-like activation of macrophages at the wound after the fin fold injury. (B) Representative image of uncut zebrafish fin fold. Maximum projection of the overlaid fluorescence of mCherry-F (macrophages) and GFP-F (tnfa+ cells). The white line outlines the fin fold and the notochord. Scale bar: 100 μm. (C) Macrophage movements and activation states were imaged by confocal microscopy from 1.5 to 9.5 hours post amputation (hpA) using the tg(mfap4:mCherry-F/tnfa:GFP-F) line. Tail images are representative maximum projections of the overlaid fluorescences of mCherry-F (macrophages) and GFP-F (tnfa⁺ cells), showing M1-like activation of recruited macrophages at the wound, starting from 3 hpA and increasing up to 7.6 hpA. Dashed line indicates the wound margin. Scale bar: 100 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Immunol