ZFIN ID: ZDB-FIG-210331-30
McGowan et al., 2021 - Wnt16 Elicits a Protective Effect Against Fractures and Supports Bone Repair in Zebrafish. JBMR plus   5:e10461 Full text @ JBMR Plus
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Anatomical Term:
Stage: Adult
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Adult

Fig 2

Bone mineralization and osteoblast recruitment is significantly delayed postfracture in wnt16−/− zebrafish. (A) Schematic illustrating fracture induction assay and labeling of old bone (Alizarin Red) and new bone (calcein green). (B) Callus formation was quantified by measuring the calcein intensity ratio between the fracture site and uninjured bone. Callus formation was significantly reduced from 2 to 7 days postinjury (dpi) in wnt16 mutant compared with WT fractures. N ≥ 5 per condition. Gray dotted line indicates where calcein intensity at the fracture site = uninjured bone. (C) Representative images of WT and wnt16−/− fish at selected time points postinjury show old bone labeled by Alizarin Red (gray) and callus formation labeled by calcein. White asterisk = center of fracture. Scale = 200 μm. (D) Representative images of calcified bone (Alizarin Red) and osteoblasts (osx:GFP) at fracture site in WT and wnt16−/− throughout fracture repair. White asterisk = center of fracture. Scale bar = 100 μm. (E) Osteoblast density was quantified by measuring the fluorescence intensity of osx:GFP within the fracture site normalized to control bone in the same fin (intensity ratio). Gray dotted line indicates where osx:GFP intensity at the fracture site = uninjured bone. Osteoblast recruitment was delayed in wnt16 mutants, which had a significantly lower osx:GFP intensity ratio at the fracture site 4 dpi, but significantly higher osx:GFP intensity ratio at 10 dpi compared with WT zebrafish. (F) Confocal imaging of bone in amputated fins at the end of the time course (15 dpi) shows complete union of fractures in both WT and wnt16−/− zebrafish. Scale bar = 100 μm. (B & E): N.s = no significant difference, *p < 0.05, **p < 0.01, ****p < 0.0001. N ≥ 6 per genotype.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP b1212Tg standard conditions Adult caudal fin principal ray bone callus IFL
b1212Tg compression injury: caudal fin Adult caudal fin principal ray bone callus IFL
wnt16bsl451/bsl451; b1212Tg standard conditions Adult caudal fin principal ray bone callus IFL
wnt16bsl451/bsl451; b1212Tg compression injury: caudal fin Adult caudal fin principal ray bone callus IFL
wnt16bsl667/bsl667; b1212Tg standard conditions Adult caudal fin principal ray bone callus IFL
wnt16bsl667/bsl667; b1212Tg compression injury: caudal fin Adult caudal fin principal ray bone callus IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT compression injury: caudal fin Adult caudal fin principal ray bone regeneration normal process quality, normal
b1212Tg compression injury: caudal fin Adult caudal fin principal ray bone callus EGFP expression increased amount, abnormal
wnt16bsl451/bsl451 compression injury: caudal fin Adult caudal fin principal ray bone regeneration decreased process quality, abnormal
wnt16bsl451/bsl451; b1212Tg compression injury: caudal fin Adult caudal fin principal ray bone callus EGFP expression increased amount, abnormal
wnt16bsl667/bsl667 compression injury: caudal fin Adult caudal fin principal ray bone regeneration decreased process quality, abnormal
wnt16bsl667/bsl667; b1212Tg compression injury: caudal fin Adult caudal fin principal ray bone callus EGFP expression increased amount, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ JBMR Plus