Figure 2

Mean normalized expression of six clock genes (orange traces): bmal1, clocka, cry1b, per1b, per2, per3 and three genes of the photoreactivation DNA repair mechanism (blue traces): cpd photolyase, cry dash, 6-4-photolyase of Amphiprion ocellaris cells, larvae and juveniles. Expression was quantified using quantitative real time PCR at zeitgeber time (h) 3, 9, 15 and 21. Lights were switched off at zeitgeber time 12, indicated by bluish-grey background. Per timepoint, three samples for larvae and juveniles and two samples for cells were analysed. The cycling expression pattern of clock genes as well as genes of the photoreactivation repair mechanism was similar between larvae and juveniles. The expression pattern of the period genes and cry1b, as well as the photolyase genes in the EAO cell line resembled those in the larvae and juveniles. However, expression of clocka and bmal1 showed a non-cycling pattern in the EAO cells compared with the high amplitude rhythmic expression observed in the animal samples.