FIGURE

Figure 2

ID

ZDB-FIG-210327-50

Publication

Marvel et al., 2021 - Gnrh2 maintains reproduction in fasting zebrafish through dynamic neuronal projection changes and regulation of gonadotropin synthesis, oogenesis, and reproductive behaviors

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Figure 2

Confocal imagery of whole tg(gnrh2:eGFP;lh:mCherry) pituitaries in normally fed (a, b) and 14-day fasted zebrafish (c, d). B’ and d’ are magnifications of the boxed regions shown in b and d. Quantification of Lh cells with close Gnrh2 neuronal contact confirms increased interactions after 14-day fasting (E). Immunohistochemistry antibody staining of Gnrh2 and Fsh on pituitary cryosections of fish in normally fed (f, g) and 14-day fasted (h, i) conditions. G’ and i’ are magnifications of the squared regions shown in g and i. Quantification of Fsh cells with close Gnrh2 contact confirms increased interactions after 14-day fasting (j). ∆ indicate close contact of Gnrh2 neurons with gonadotropes. All data expressed as means ± S.E.M, n = 6 pituitaries per group, *P < 0.05.

Expression Data

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Antibody Labeling

Phenotype Data

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.