ZFIN ID: ZDB-FIG-210310-65
Lu et al., 2021 - Generation of Knockout and Transgenic Zebrafish to Characterize Abcc4 Functions in Detoxification and Efflux of Lead. International Journal of Molecular Sciences   22(4) Full text @ Int. J. Mol. Sci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Anatomical Terms:
Stage Range: Day 5 to Adult
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Day 5 to Adult

Figure 1 Lead (Pb)-induced expression of the abcc4 gene in developing embryos and adult tissues of zebrafish. (A) Induced expression of the zebrafish abcc4 gene in developing embryos under lead stress. Embryos were treated with 5 μM lead from 24 to 120 h post-fertilization (hpf) and subjected to whole-mount RNA in situ hybridization (WISH) with antisense RNA probe. (a,b) Lateral views; (c,d) dorsal views. Yellow, white and red dashed lines indicate the oral cavity, gill and pronephric tubules, respectively. (B) Transcriptional responses of the zebrafish abcc4 gene to lead treatments in adult tissues. Adult zebrafish were exposed to various concentrations of lead for 24 h. Gills and intestines were taken from four females, and kidneys were taken from four females and four males. The same tissues from different fish were mixed for isolation of total RNA and subjected to qRT-PCR analysis of abcc4 mRNA levels relative to the control. One-way analysis of variance (ANOVA) followed by Duncan’s post-hoc test was performed, and the symbol above the error bars indicates a significant difference (* p < 0.05 and ** p < 0.01) of mRNA levels in the same tissue among the indicated doses of treatments.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
abcc4 AB chemical treatment by environment: lead(0) Day 5 gill ISH
Day 5 oral cavity ISH
Day 5 pronephric tubule ISH
Adult intestine RTPCR
Adult kidney RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB chemical treatment by environment: lead(0) Day 5 gill abcc4 expression increased amount, abnormal
Day 5 oral cavity abcc4 expression increased amount, abnormal
Day 5 pronephric tubule abcc4 expression increased amount, abnormal
Adult intestine abcc4 expression increased amount, abnormal
Adult kidney abcc4 expression increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal International Journal of Molecular Sciences for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Int. J. Mol. Sci.