FIGURE

Fig. 3

ID
ZDB-FIG-210208-58
Publication
Zaksauskaite et al., 2021 - Tdp1 protects from topoisomerase 1-mediated chromosomal breaks in adult zebrafish but is dispensable during larval development
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Fig. 3

<italic>Tdp1<sup>−/−</sup></italic> fish have a mild locomotion defect in adulthood but not at 4 to 5 dpf.

(A to F) Adult zebrafish movement was recorded with a camera system for 6 hours. Time spent swimming at low (<30 mm/s) (A), medium (30 to 60 mm/s) (C), and high (E) speeds (60 mm/s or above) and count of times low (B), medium (D), and high (F) swimming speeds were plotted. Each of the 18 fish from either genotype was recorded twice (n = 36) and shown as average ± SEM. P values were calculated by two-tailed Student’s t test with Holm adjustment for multiple comparisons. (G to J) Four-dpf (G and H) and 5-dpf (I and J) embryos from a single female tdp1−/− and male tdp1−/+ incross were subjected to 3 cycles of 5-min darkness and 5-min light using the photomotor response assay. (G and I) Total distance traveled in each cycle was plotted in each data point as average ± SEM. P value, two-tailed Student’s t test. (H and J) Total distance traveled in all light or dark cycles was measured. Lines indicate average ± SEM. (I) Total distance traveled each cycle was plotted as average ± SEM. P values were calculated using two-tailed Student’s t test. DMSO, dimethyl sulfoxide. *P < 0.05; **P < 0.01.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Condition:
Observed In:
Stage Range: Day 4 to Adult

Phenotype Detail
Acknowledgments
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