FIGURE

Fig. 1

ID

ZDB-FIG-210201-1

Publication

Kula-Alwar et al., 2020 - Mef2c factors are required for early but not late addition of cardiomyocytes to the ventricle

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Fig. 1

Ventricular myocardium expands and thickens by CM addition. (A) Ventricular axes were measured on confocal images of live Tg(myl7:EGFP) embryonic hearts at 3 and 5 dpf. Ventral half of ventricle shown. Graph shows increase in length of each axis. (B) Myocardial thickness increased significantly from 3 to 5 dpf. (C,D) Myocardial volume (C) and surface area (D) were measured by thresholding for GFP in scans of Tg(myl7:EGFP) embryos (see materials and methods), both increased significantly by 52% between 3 and 5 dpf. (E) Trabecular length increased 500% between 3 and 5 dpf. (F)Tg(myl7:EGFP);Tg(myl7:DsRed2-NLS) were thresholded to count DsRed CM nuclei. Number of ventricular CMs increased by 26% from 3 to 5 dpf, n = 5 and n = 4 respectively. (G,H) Confocal section through Tg(myl7:EGFP-Has.HRAS)^s883 scatter labelled with cytoplasmic mCherry (G, white arrow). Graph of average cell volume (H) measured from three hearts at 3 dpf (9 cells) or 5 dpf (10 cells). (I,J) Confocal optical sections through Tg(myl7:EGFP-Has.HRAS)^s883 fish at 3 and 5 dpf with four CM perimeter measures carried out in each optical plane. Graph (J) shows cell measurements from four hearts for each time point. Values in all graphs are mean ± SEM. N for each group shown on bars. Statistics used unpaired Student’s t-test. Scale bars = 20 μm.

Expression Data

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Antibody Labeling

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Acknowledgments

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Reprinted from Developmental Biology, 470, Kula-Alwar, D., Marber, M.S., Hughes, S.M., Hinits, Y., Mef2c factors are required for early but not late addition of cardiomyocytes to the ventricle, 95-107, Copyright (2020) with permission from Elsevier. Full text @ Dev. Biol.