Figure 4—figure supplement 1.

4T1 extracellular vesicles (EVs) organotropism.

(a) Organs (left) and lungs sections (right) harvested from mice injected with PBS-MB or 4T1 MB-EVs 1 hr after intravenous injection. Scale bars: 10 μm. (b) Representative confocal images showing the identity of lung cells accumulating fluorescently labeled 4T1 EVs. Scale bars: 10 μm. Graphs show the percentage of endothelial cells, fibroblasts, macrophages and cells of unknown identity among EV-positive cells (108 positive cells from six mice taken from two independent experiments). (c) Graph showing liver accumulation of fluorescent EVs isolated from shControl, shRalA, and shRalB cells. Equal numbers of EVs were injected intravenously 1 hr before organ harvesting (eight mice taken from two independent experiments; Kruskal-Wallis test followed by Dunn's Multiple Comparison Test).

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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